Hotspot

psbM-trnD-GUC

LSC · 30010-30749 · 740 bp

Back to Tashiroea

Pi 0.0148

MarkerSeek score 57.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 25
pi: 0.0148
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGCGAAGTGGACTCTAAAGG	CCCAAGAATTCCCTGATCCG	55.0	55.0	58.0	58.0	1757-1778 bp	1.0	83.5
psbM-trnD-GUC_p2	GGGCGAAGTGGACTCTAAAG	CCCAAGAATTCCCTGATCCG	55.0	55.0	58.0	58.0	1758-1779 bp	1.0	83.5
psbM-trnD-GUC_p3	TCTTGCTTGGGCTGCTTTAA	CCCAAGAATTCCCTGATCCG	45.0	55.0	58.0	58.0	1816-1837 bp	1.0	83.4
psbM-trnD-GUC_p4	TGACTATGCGCCATTGATCC	CCCAAGAATTCCCTGATCCG	50.0	55.0	58.1	58.0	1909-1930 bp	1.0	82.3
psbM-trnD-GUC_p5	TTTCATCAACGGCGTTAGGT	CCCAAGAATTCCCTGATCCG	45.0	55.0	57.8	58.0	1947-1967 bp	1.0	81.8