Hotspot

rpoB-trnC-GCA

LSC · 27072-28516 · 1445 bp

Back to Tashiroea

Pi 0.0135

MarkerSeek score 58.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1445
variable_sites: 39
pi: 0.0135
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	GGGGAATGCGCTGAATCTTA	50.0	50.0	58.0	58.0	2635-2667 bp	1.0	84.4
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	ACACCCCATTACGTAGTGGA	50.0	50.0	58.0	58.1	2249-2292 bp	1.0	84.3
rpoB-trnC-GCA_p3	AGAAAATACGGGATCGCCAC	GGGGAATGCGCTGAATCTTA	50.0	50.0	58.1	58.0	2843-2875 bp	1.0	83.9
rpoB-trnC-GCA_p4	CAGAAAATACGGGATCGCCA	GGGGAATGCGCTGAATCTTA	50.0	50.0	58.1	58.0	2844-2876 bp	1.0	83.9
rpoB-trnC-GCA_p5	AGAAAATACGGGATCGCCAC	ACACCCCATTACGTAGTGGA	50.0	50.0	58.1	58.1	2457-2500 bp	1.0	83.7