Hotspot

trnR-UCU-atpA

LSC · 9828-10133 · 306 bp

Back to Tashiroea

Pi 0.016

MarkerSeek score 58.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 306
variable_sites: 13
pi: 0.016
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTCACCGAGGAAGCAGAA	47.6	50.0	57.3	57.9	394-409 bp	1.0	70.7
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAGACATTCACCGAGGAAGC	47.6	50.0	57.3	57.9	398-413 bp	1.0	70.6
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	CCGAGGAAGCAGAATCCATT	47.6	50.0	57.3	57.7	388-403 bp	1.0	68.8
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TTCACCGAGGAAGCAGAATC	47.6	50.0	57.3	57.6	392-407 bp	1.0	68.4
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	GACATTCACCGAGGAAGCAG	47.6	55.0	57.3	58.6	396-411 bp	1.0	66.3