Hotspot

matK-trnK-UUU

LSC · 3451-4210 · 760 bp

Back to Tashiroea

Pi 0.0124

MarkerSeek score 60.40

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 24
pi: 0.0124
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGGAAGTTGTGTTGCCAAGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.6	58.2	832-841 bp	1.0	78.3
matK-trnK-UUU_p2	AGGAAGTTGTGTTGCCAAGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.6	58.2	831-840 bp	1.0	78.3
matK-trnK-UUU_p3	AGGAAGTTGTGTTGCCAAGA	TTGGGTTGCTAACTCAACGG	45.0	50.0	57.6	58.4	847-856 bp	1.0	76.4
matK-trnK-UUU_p4	AGGAAGTTGTGTTGCCAAGA	GGGTTGCTAACTCAACGGTA	45.0	50.0	57.6	57.5	845-854 bp	1.0	75.9
matK-trnK-UUU_p5	AGGAAGTTGTGTTGCCAAGAT	ACGGTAGAGTACTCGGCTTT	42.9	50.0	58.1	58.2	831-840 bp	1.0	73.4