Hotspot

trnH-GUG-psbA

LSC · 76-304 · 229 bp

Back to Tashiroea

Pi 0.0631
MarkerSeek score 71.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
229
variable_sites
27
pi
0.0631
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCTGTTGAAGTTCCATCTACA 55.0 42.9 58.1 55.9 278-281 bp 1.0 63.9
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC GCTGTTGAAGTTCCATCTACA 50.0 42.9 57.9 55.9 291-294 bp 1.0 63.5
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GCTGTTGAAGTTCCATCTACA 50.0 42.9 57.9 55.9 292-295 bp 1.0 63.4
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCTGTTGAAGTTCCATCTACA 50.0 42.9 57.9 55.9 293-296 bp 1.0 63.4
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GCTGTTGAAGTTCCATCTACAA 55.0 40.9 58.1 56.6 278-281 bp 1.0 61.8