Hotspot

ndhF

SSC · 108472-110691 · 2220 bp

Back to Taraxacum

Pi 0.00549

MarkerSeek score 42.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 29
pi: 0.00549
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCCGGAACTTCTTCGTAGTG	AACGAGGTAAGATCCGGTGA	55.0	50.0	58.0	58.2	2980-2997 bp	1.0	80.3
ndhF_p2	CCCGGAACTTCTTCGTAGTG	ACGAGGTAAGATCCGGTGAA	55.0	50.0	58.0	58.2	2979-2996 bp	1.0	80.3
ndhF_p3	CCGGAACTTCTTCGTAGTGG	AACGAGGTAAGATCCGGTGA	55.0	50.0	58.0	58.2	2979-2996 bp	1.0	80.3
ndhF_p4	CCGGAACTTCTTCGTAGTGG	ACGAGGTAAGATCCGGTGAA	55.0	50.0	58.0	58.2	2978-2995 bp	1.0	80.3
ndhF_p5	CCGGAACTTCTTCGTAGTGG	GAACGAGGTAAGATCCGGTG	55.0	55.0	58.0	57.8	2980-2997 bp	1.0	79.9