Hotspot

rps15-ycf1

SSC · 122027-122439 · 413 bp

Back to Taraxacum

Pi 0.00636
MarkerSeek score 54.80
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
413
variable_sites
6
pi
0.00636
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGTCTCCGTATCTTATTGGTGA GCAGCCGCTTGAGTATGTTA 40.9 50.0 56.1 58.3 548-617 bp 1.0 49.7
rps15-ycf1_p2 AAGTCTCCGTATCTTATTGGTGA GCAGCCGCTTGAGTATGTTA 39.1 50.0 56.8 58.3 549-618 bp 1.0 47.3
rps15-ycf1_p3 AGTCTCCGTATCTTATTGGTGAA GCAGCCGCTTGAGTATGTTA 39.1 50.0 56.8 58.3 548-617 bp 1.0 47.3
rps15-ycf1_p4 GTCTCCGTATCTTATTGGTGA GCAGCCGCTTGAGTATGTTA 42.9 50.0 54.6 58.3 547-616 bp 1.0 45.5
rps15-ycf1_p5 AGTCTCCGTATCTTATTGGTG GCAGCCGCTTGAGTATGTTA 42.9 50.0 54.6 58.3 548-617 bp 1.0 45.4