Hotspot

ndhA-ndhA

SSC · 118868-119921 · 1054 bp

Back to Taraxacum

Pi 0.00497

MarkerSeek score 57.40

Primer available yes

Label ndhA-ndhA

Feature Metadata

label_name: ndhA-ndhA
feature_type: igs
region: SSC
length_bp: 1054
variable_sites: 13
pi: 0.00497
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhA_p1	AAAGCCTATAGGTTGACGCC	TTAGGTGGTCTACGAGCTGC	50.0	55.0	57.7	59.2	1224-1235 bp	1.0	68.9
ndhA-ndhA_p2	AAAGCCTATAGGTTGACGCC	TTTAGGTGGTCTACGAGCTG	50.0	50.0	57.7	56.7	1225-1236 bp	1.0	67.8
ndhA-ndhA_p3	AAAGCCTATAGGTTGACGCC	TAGGTGGTCTACGAGCTGC	50.0	57.9	57.7	58.5	1223-1234 bp	1.0	66.3
ndhA-ndhA_p4	CCTATAGGTTGACGCCACAA	TTAGGTGGTCTACGAGCTGC	50.0	55.0	57.3	59.2	1220-1231 bp	1.0	66.0
ndhA-ndhA_p5	GTTGACGCCACAAATTCCAT	TTAGGTGGTCTACGAGCTGC	45.0	55.0	57.3	59.2	1213-1224 bp	1.0	65.7