Hotspot

ycf4-cemA

LSC · 60335-61124 · 790 bp

Back to Taraxacum

Pi 0.00567

MarkerSeek score 57.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 11
pi: 0.00567
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	970-979 bp	1.0	80.1
ycf4-cemA_p2	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	934-943 bp	1.0	79.6
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	902-911 bp	1.0	79.6
ycf4-cemA_p4	TTGCGTGTACCGATTGAAGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	890-899 bp	1.0	79.1
ycf4-cemA_p5	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	916-925 bp	1.0	78.9