Hotspot

rbcL-psaI

LSC · 56496-59259 · 2764 bp

Back to Taraxacum

Pi 0.00396

MarkerSeek score 45.40

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 2764
variable_sites: 28
pi: 0.00396
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AGGCTACCAAATGGAGTCCT	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	2700-2950 bp	1.0	84.6
rbcL-psaI_p2	GGCTACCAAATGGAGTCCTG	AAAGAAGCCATTGCGATTGC	55.0	45.0	58.0	58.0	2699-2949 bp	1.0	84.5
rbcL-psaI_p3	AGGCTACCAAATGGAGTCCT	TTGCGATTGCCGGAAATACT	50.0	45.0	58.0	58.3	2690-2940 bp	1.0	82.6
rbcL-psaI_p4	GAGTCCTGAACTAGCTGCTG	AAAGAAGCCATTGCGATTGC	55.0	45.0	57.7	58.0	2687-2937 bp	1.0	82.5
rbcL-psaI_p5	GGCTACCAAATGGAGTCCTG	TTGCGATTGCCGGAAATACT	55.0	45.0	58.0	58.3	2689-2939 bp	1.0	82.4