Hotspot

ndhC-trnV-UAC

LSC · 50054-51265 · 1212 bp

Back to Taraxacum

Pi 0.00691

MarkerSeek score 61.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1212
variable_sites: 22
pi: 0.00691
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTTGCTACTCGGAGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1332-1359 bp	1.0	81.3
ndhC-trnV-UAC_p2	ACTCGGAGCTAAAAATCCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1323-1350 bp	1.0	79.9
ndhC-trnV-UAC_p3	TTCTCCGGTCCTTTGCTACT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.4	58.1	1340-1367 bp	1.0	79.5
ndhC-trnV-UAC_p4	TTTCTCCGGTCCTTTGCTAC	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.5	58.1	1341-1368 bp	1.0	78.6
ndhC-trnV-UAC_p5	TCCTTTGCTACTCGGAGCTA	AGCCCAATGCACAAAAATGC	50.0	45.0	57.9	58.5	1433-1438 bp	0.571429	58.6