Hotspot

ycf3-trnS-GGA

LSC · 43873-44789 · 917 bp

Back to Taraxacum

Pi 0.00156

MarkerSeek score 55.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 917
variable_sites: 4
pi: 0.00156
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	1020-1031 bp	1.0	72.1
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	970-981 bp	1.0	71.9
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	47.6	55.0	58.0	57.8	1008-1019 bp	1.0	71.6
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	972-983 bp	1.0	71.3
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	47.6	50.0	58.0	57.6	998-1009 bp	1.0	70.2