Hotspot

ycf1

SSC · 125031-130622 · 5592 bp

Back to Tapeinosperma

Pi 0.00218

MarkerSeek score 57.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5592
variable_sites: 11
pi: 0.00218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGATGCAACCCATTCAAGA	TGAAACGAAAGAGAGCCGAG	45.0	50.0	57.8	57.9	607 bp	0.5	57.9
ycf1_p2	ACGATGCAACCCATTCAAGA	GAAACGAAAGAGAGCCGAGT	45.0	50.0	57.8	57.9	606 bp	0.5	57.9
ycf1_p3	CACGATGCAACCCATTCAAG	TGAAACGAAAGAGAGCCGAG	50.0	50.0	57.7	57.9	608 bp	0.5	57.3
ycf1_p4	CACGATGCAACCCATTCAAG	GAAACGAAAGAGAGCCGAGT	50.0	50.0	57.7	57.9	607 bp	0.5	57.3
ycf1_p5	ACGATGCAACCCATTCAAGA	AAACGAAAGAGAGCCGAGTG	45.0	50.0	57.8	58.2	605 bp	0.5	56.7