Hotspot

rps16

LSC · 5019-6131 · 1113 bp

Back to Tapeinosperma

Pi 0.0018

MarkerSeek score 59.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1113
variable_sites: 2
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGTAGGGAAGTGATGACTCGT	TCAATTCCATACCGGTTCCA	47.6	45.0	57.9	56.5	1225 bp	1.0	59.6
rps16_p2	AGTAGGGAAGTGATGACTCGT	TCAATTCCATACCGGTTCCAA	47.6	42.9	57.9	57.3	1225 bp	1.0	57.6
rps16_p3	AGTAGGGAAGTGATGACTCGT	ATCAATTCCATACCGGTTCCA	47.6	42.9	57.9	57.1	1226 bp	1.0	55.9
rps16_p4	GTAGGGAAGTGATGACTCGT	TCAATTCCATACCGGTTCCA	50.0	45.0	56.4	56.5	1224 bp	1.0	55.3
rps16_p5	AGTAGGGAAGTGATGACTCGT	TCAATTCCATACCGGTTCCAAA	47.6	40.9	57.9	58.0	1225 bp	1.0	55.1