Hotspot

rpoC2

LSC · 17111-21295 · 4185 bp

Back to Tapeinosperma

Pi 0.000717

MarkerSeek score 51.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4185
variable_sites: 3
pi: 0.000717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTCTCGTGGAGAATGGCAAG	GATGTGTATCATGCGCCTGA	50.0	50.0	57.9	58.1	332 bp	1.0	78.6
rpoC2_p2	TTCTCGTGGAGAATGGCAAG	TACCGATGTGTATCATGCGC	50.0	50.0	57.9	58.2	336 bp	1.0	77.9
rpoC2_p3	TTCTCGTGGAGAATGGCAAG	ATGTGTATCATGCGCCTGAA	50.0	45.0	57.9	57.7	331 bp	1.0	77.0
rpoC2_p4	TTCTCGTGGAGAATGGCAAG	TGTGTATCATGCGCCTGAAT	50.0	45.0	57.9	57.7	330 bp	1.0	77.0
rpoC2_p5	TCGTGGAGAATGGCAAGATC	GATGTGTATCATGCGCCTGA	50.0	50.0	57.7	58.1	329 bp	1.0	76.9