Hotspot

rbcL

LSC · 56072-57499 · 1428 bp

Back to Tapeinosperma

Pi 0.0021

MarkerSeek score 60.00

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 3
pi: 0.0021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCGAGTAGACCTTGTTGTTGT	CCTCTAGCCAAAGTGGATGC	42.9	55.0	56.9	58.3	1600 bp	1.0	60.8
rbcL_p2	CGAGTAGACCTTGTTGTTGTG	CCTCTAGCCAAAGTGGATGC	47.6	55.0	56.9	58.3	1599 bp	1.0	60.5
rbcL_p3	TCGAGTAGACCTTGTTGTTGT	CTCTAGCCAAAGTGGATGCA	42.9	50.0	56.9	57.6	1599 bp	1.0	60.2
rbcL_p4	CGAGTAGACCTTGTTGTTGTG	CTCTAGCCAAAGTGGATGCA	47.6	50.0	56.9	57.6	1598 bp	1.0	59.8
rbcL_p5	GTCGAGTAGACCTTGTTGTTGT	CCTCTAGCCAAAGTGGATGC	45.5	55.0	58.3	58.3	1601 bp	1.0	59.3