Hotspot

ndhF

SSC · 111337-113559 · 2223 bp

Back to Tapeinosperma

Pi 0.0027

MarkerSeek score 48.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 6
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGAAACGAAAGAGAGCCGAG	ACCTATTGATTCGAAAACAATTCAGA	50.0	30.8	57.9	57.7	2369 bp	1.0	40.2
ndhF_p2	GAAACGAAAGAGAGCCGAGT	ACCTATTGATTCGAAAACAATTCAGA	50.0	30.8	57.9	57.7	2368 bp	1.0	40.2
ndhF_p3	AAACGAAAGAGAGCCGAGTG	ACCTATTGATTCGAAAACAATTCAGA	50.0	30.8	58.2	57.7	2367 bp	1.0	40.2
ndhF_p4	CGAAAGAGAGCCGAGTGAAT	ACCTATTGATTCGAAAACAATTCAGA	50.0	30.8	57.7	57.7	2364 bp	1.0	40.2
ndhF_p5	TGAAACGAAAGAGAGCCGAG	ACCTATTGATTCGAAAACAATTCAG	50.0	32.0	57.9	56.4	2369 bp	1.0	40.2