Hotspot

ccsA-ndhD

SSC · 116549-116797 · 249 bp

Back to Tapeinosperma

Pi 0.0442

MarkerSeek score 77.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 249
variable_sites: 11
pi: 0.0442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAGGAATCGGGCTACACAGT	TCAGTTGACAAGGTCGAAGT	50.0	45.0	57.8	56.7	374 bp	1.0	71.0
ccsA-ndhD_p2	CGGGCTACACAGTTATGGTT	TCAGTTGACAAGGTCGAAGT	50.0	45.0	57.6	56.7	367 bp	1.0	69.1
ccsA-ndhD_p3	TAGGAATCGGGCTACACAGT	TCAGTTGACAAGGTCGAAGTT	50.0	42.9	57.8	57.5	374 bp	1.0	68.8
ccsA-ndhD_p4	TCGGGCTACACAGTTATGGT	TCAGTTGACAAGGTCGAAGT	50.0	45.0	58.4	56.7	368 bp	1.0	68.7
ccsA-ndhD_p5	TGTTGTGGCTTCTATTGGCT	TCAGTTGACAAGGTCGAAGT	45.0	45.0	57.4	56.7	433 bp	1.0	67.2