Hotspot

ndhF-rpl32

SSC · 113560-114527 · 968 bp

Back to Tapeinosperma

Pi 0.0108

MarkerSeek score 69.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 968
variable_sites: 10
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GACGAAGCCTTTTTGTTGGG	AAAGCCGCCCAATATCCTTG	50.0	50.0	57.9	58.6	1121-1166 bp	1.0	78.0
ndhF-rpl32_p2	GACGAAGCCTTTTTGTTGGG	CCGCCCAATATCCTTGCTTT	50.0	50.0	57.9	58.6	1117-1162 bp	1.0	78.0
ndhF-rpl32_p3	CGACGAAGCCTTTTTGTTGG	AAAGCCGCCCAATATCCTTG	50.0	50.0	58.2	58.6	1122-1167 bp	1.0	77.1
ndhF-rpl32_p4	CGACGAAGCCTTTTTGTTGG	CCGCCCAATATCCTTGCTTT	50.0	50.0	58.2	58.6	1118-1163 bp	1.0	77.1
ndhF-rpl32_p5	CCACATACGACGAAGCCTTT	CCGCCCAATATCCTTGCTTT	50.0	50.0	58.3	58.6	1125-1170 bp	1.0	76.8