Hotspot

psaA-ycf3

LSC · 43083-43746 · 664 bp

Back to Tapeinosperma

Pi 0.00151

MarkerSeek score 59.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 664
variable_sites: 1
pi: 0.00151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	842-852 bp	1.0	78.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	838-848 bp	1.0	77.1
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	840-850 bp	1.0	77.1
psaA-ycf3_p4	TTGAGAAATGACCCGGTCTG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.5	58.0	862-872 bp	1.0	76.0
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	795-805 bp	1.0	75.9