Hotspot

trnR-UCU-atpA

LSC · 11013-11136 · 124 bp

Back to Tapeinosperma

Pi 0.0161

MarkerSeek score 61.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 2
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	CGGAATGAATGAAAAGCGTCC	GCTATTCAGGAACAGACGGA	47.6	50.0	58.2	57.0	259-268 bp	1.0	63.2
trnR-UCU-atpA_p2	CGGAATGAATGAAAAGCGTCC	AGCTATTCAGGAACAGACGG	47.6	50.0	58.2	57.0	260-269 bp	1.0	63.1
trnR-UCU-atpA_p3	CGGAATGAATGAAAAGCGTCC	GGAAGCCCTTTTGAAAGAAGC	47.6	47.6	58.2	58.0	278-287 bp	1.0	62.6
trnR-UCU-atpA_p4	CCAAACAAGAGGCAAAATCGG	GCTATTCAGGAACAGACGGA	47.6	50.0	58.3	57.0	277-286 bp	1.0	62.3
trnR-UCU-atpA_p5	CCAAACAAGAGGCAAAATCGG	AGCTATTCAGGAACAGACGG	47.6	50.0	58.3	57.0	278-287 bp	1.0	62.2