Hotspot

psbK-psbI

LSC · 8519-8912 · 394 bp

Back to Tapeinosperma

Pi 0.00508

MarkerSeek score 57.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 2
pi: 0.00508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GCTGCTGTAAGTTTTCGCTG	AGGATTACGCCCTGGATCAT	50.0	50.0	58.0	58.3	511 bp	1.0	77.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	AGGATTACGCCCTGGATCAT	50.0	50.0	57.9	58.3	520 bp	1.0	77.7
psbK-psbI_p3	AGCTGCTGTAAGTTTTCGCT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.1	58.3	512 bp	1.0	77.3
psbK-psbI_p4	TATGCCAGTCATCCCTCTGT	AGGATTACGCCCTGGATCAT	50.0	50.0	57.8	58.3	560 bp	1.0	76.7
psbK-psbI_p5	GCCAGTCATCCCTCTGTTTT	AGGATTACGCCCTGGATCAT	50.0	50.0	57.8	58.3	557 bp	1.0	76.5