Hotspot

rps18

LSC · 69070-69666 · 597 bp

Back to Tanaecium

Pi 0.0325

MarkerSeek score 69.40

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 597
variable_sites: 16
pi: 0.0325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AAAGGAGGTTCATGGCCAAG	GACCAGAACTAAACGCGGAT	50.0	50.0	58.1	58.0	1792-2512 bp	1.0	85.1
rps18_p2	AAGAAGAAAGATGCGCGAGT	GACCAGAACTAAACGCGGAT	45.0	50.0	57.9	58.0	1775-2495 bp	1.0	85.0
rps18_p3	AGGTGTCAACGGGAATTTCC	GACCAGAACTAAACGCGGAT	50.0	50.0	58.1	58.0	1708-2428 bp	1.0	85.0
rps18_p4	AAAGGAGGTTCATGGCCAAG	TATCAAGTTTTCGAGGGGCG	50.0	50.0	58.1	58.0	2453 bp	0.5	59.3
rps18_p5	AAGAAGAAAGATGCGCGAGT	TATCAAGTTTTCGAGGGGCG	45.0	50.0	57.9	58.0	2436 bp	0.5	59.2