Hotspot

accD

LSC · 57327-59075 · 1749 bp

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Pi 0.0479

MarkerSeek score 75.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1749
variable_sites: 79
pi: 0.0479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GGAGTCTTGAACTAGCTGCC	AAAGAAGCCATTGCCATTGC	55.0	45.0	58.0	57.9	2690-2797 bp	1.0	85.4
accD_p2	ACTACAGTTCGGTGGAGGAA	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.0	57.9	2845-2952 bp	1.0	85.3
accD_p3	GGAGTCTTGAACTAGCTGCC	TTGCCATTGCCGGAAATACT	55.0	45.0	58.0	58.2	2680-2787 bp	1.0	85.0
accD_p4	ACTACAGTTCGGTGGAGGAA	TTGCCATTGCCGGAAATACT	50.0	45.0	58.0	58.2	2835-2942 bp	1.0	84.9
accD_p5	GCTCTGACCGAGATCTTTGG	AAAGAAGCCATTGCCATTGC	55.0	45.0	58.1	57.9	2877-2984 bp	1.0	84.8