Hotspot

trnN-GUU-ycf1

IRb · 109427-110252 · 826 bp

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Pi 0.04

MarkerSeek score 71.60

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 826
variable_sites: 24
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	AGGATTCGAACCTACGACCA	ACGACAACGACCGAATTGAT	50.0	45.0	58.2	57.9	740-965 bp	1.0	84.4
trnN-GUU-ycf1_p2	CCCCAAGTAGGATTCGAACC	ACGACAACGACCGAATTGAT	55.0	45.0	57.7	57.9	748-973 bp	1.0	83.0
trnN-GUU-ycf1_p3	AGGATTCGAACCTACGACCA	TCCATAATAGAGCCCGACGA	50.0	50.0	58.2	57.7	756-981 bp	1.0	82.7
trnN-GUU-ycf1_p4	AGGATTCGAACCTACGACCA	CCATAATAGAGCCCGACGAC	50.0	55.0	58.2	57.7	755-980 bp	1.0	82.2
trnN-GUU-ycf1_p5	CCCCAAGTAGGATTCGAACC	TCCATAATAGAGCCCGACGA	55.0	50.0	57.7	57.7	764-989 bp	1.0	81.3