Hotspot

rps12-clpP

LSC · 71181-71364 · 184 bp

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Pi 0.0489
MarkerSeek score 66.00
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
184
variable_sites
9
pi
0.0489
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 TCATTCATACCGGAAAGCGG CGCGACTCCATCATAGATGG 50.0 55.0 58.1 58.0 524-583 bp 1.0 82.5
rps12-clpP_p2 TCTTTGGCTCTACCCCTGAA TCTGTTCGTTTCACACAGCA 50.0 45.0 58.0 58.0 1012-1069 bp 1.0 81.9
rps12-clpP_p3 TCTTTGGCTCTACCCCTGAA TGTTCGTTTCACACAGCAGA 50.0 45.0 58.0 58.0 1010-1067 bp 1.0 81.9
rps12-clpP_p4 TCTTTGGCTCTACCCCTGAA CGCGACTCCATCATAGATGG 50.0 55.0 58.0 58.0 883-940 bp 1.0 81.6
rps12-clpP_p5 TCTTTGGCTCTACCCCTGAA TAGAAAGCAACGGAGCCATC 50.0 50.0 58.0 58.0 1240-1297 bp 1.0 81.6