Hotspot

rpl33-rps18

LSC · 67976-69069 · 1094 bp

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Pi 0.0522

MarkerSeek score 70.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 25
pi: 0.0522
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.44
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ATTCAACGTCTGCGAGACTC	AAAACACCAATCCGCGTTTG	50.0	45.0	58.0	58.2	1552-2282 bp	1.0	84.4
rpl33-rps18_p2	ATTCAACGTCTGCGAGACTC	CAAAACACCAATCCGCGTTT	50.0	45.0	58.0	58.2	1553-2283 bp	1.0	84.4
rpl33-rps18_p3	GATTCAACGTCTGCGAGACT	AAAACACCAATCCGCGTTTG	50.0	45.0	58.0	58.2	1553-2283 bp	1.0	84.4
rpl33-rps18_p4	GATTCAACGTCTGCGAGACT	CAAAACACCAATCCGCGTTT	50.0	45.0	58.0	58.2	1554-2284 bp	1.0	84.4
rpl33-rps18_p5	AAAGGAGGTTCATGGCCAAG	AAAACACCAATCCGCGTTTG	50.0	45.0	58.1	58.2	1245-1965 bp	1.0	84.2