Hotspot

petA-psbJ

LSC · 63287-64285 · 999 bp

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Pi 0.014

MarkerSeek score 66.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 14
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1056-1059 bp	1.0	75.8
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1058-1061 bp	1.0	75.8
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1055-1058 bp	1.0	75.3
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1057-1060 bp	1.0	75.3
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	1055-1058 bp	1.0	74.9