Hotspot

psaI-ycf4

LSC · 59505-60016 · 512 bp

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Pi 0.0692

MarkerSeek score 77.20

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 33
pi: 0.0692
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TAGGCCTAGTATTTCCGGCA	TTGCATTTTTCCCTCCTGCT	50.0	45.0	57.9	58.0	1127-1180 bp	1.0	87.6
psaI-ycf4_p2	GCAATGGCAATGGCTTCTTT	CCAGGAAATTGTGGACCACA	45.0	50.0	57.9	58.0	754-788 bp	1.0	87.1
psaI-ycf4_p3	TAGGCCTAGTATTTCCGGCA	CCAGGAAATTGTGGACCACA	50.0	50.0	57.9	58.0	771-805 bp	1.0	87.0
psaI-ycf4_p4	TTTGAAGTTCCACGGCTTCA	TTGCATTTTTCCCTCCTGCT	45.0	45.0	58.0	58.0	1587 bp	1.0	86.0
psaI-ycf4_p5	TTTGAAGTTCCACGGCTTCA	CCAGGAAATTGTGGACCACA	45.0	50.0	58.0	58.0	1212-1231 bp	1.0	85.2