Hotspot

rbcL-accD

LSC · 56873-57326 · 454 bp

Back to Tanaecium

Pi 0.0463

MarkerSeek score 67.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 21
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACTACAGTTCGGTGGAGGAA	GACATTGCCGGGGTGATATT	50.0	50.0	58.0	58.0	917 bp	0.5	59.8
rbcL-accD_p2	ACTACAGTTCGGTGGAGGAA	GCCGGGGTGATATTTGTCAT	50.0	50.0	58.0	58.0	911 bp	0.5	59.8
rbcL-accD_p3	GGAGTCTTGAACTAGCTGCC	GACATTGCCGGGGTGATATT	55.0	50.0	58.0	58.0	762 bp	0.5	59.7
rbcL-accD_p4	GGAGTCTTGAACTAGCTGCC	GCCGGGGTGATATTTGTCAT	55.0	50.0	58.0	58.0	756 bp	0.5	59.7
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	GCCGGGGTGATATTTGTCAT	50.0	50.0	58.0	58.0	1390 bp	0.5	59.7