primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	7827	8208	488-502	1.000000	82.6
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	7827	8208	489-503	1.000000	82.6
psbK-psbI_p3	psbK-psbI	3	CTTTTGTTTGGCAAGCTGCT	ACGCCCCGGATCATTAGATA	20	20	45.0	50.0	58.1	58.1	15.2	0.7	0.122947	7827	8208	493-507	1.000000	82.5
psbK-psbI_p4	psbK-psbI	4	CTTTTGTTTGGCAAGCTGCT	TACGCCCCGGATCATTAGAT	20	20	45.0	50.0	58.1	58.1	15.2	0.7	0.122947	7827	8208	494-508	1.000000	82.5
psbK-psbI_p5	psbK-psbI	5	GCCAGTCATACCTGTGCTTT	ACGCCCCGGATCATTAGATA	20	20	50.0	50.0	58.2	58.1	0.0	0.7	0.240594	7827	8208	525-539	1.000000	81.4
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TTGCTTCTCCTGATGGTTGG	TCGAACCCACATCTTCTCCT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.266438	35889	36153	431-458	1.000000	80.0
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	GCTTCTCCTGATGGTTGGTC	TCGAACCCACATCTTCTCCT	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.317420	35889	36153	429-456	1.000000	79.6
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	CCGTCGTATTTGCTTCTCCT	TCGAACCCACATCTTCTCCT	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.356668	35889	36153	440-467	1.000000	79.3
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	TTGCTTCTCCTGATGGTTGG	CCACATCTTCTCCTTGGCAA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.410045	35889	36153	425-452	1.000000	78.9
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	GCTTCTCCTGATGGTTGGTC	CCACATCTTCTCCTTGGCAA	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.461026	35889	36153	423-450	1.000000	78.5
rbcL-accD_p1	rbcL-accD	1	ACTACAGTTCGGTGGAGGAA	GACATTGCCGGGGTGATATT	20	20	50.0	50.0	58.0	58.0	0.0	2.2	0.024114	56873	57326	917-917	0.500000	59.8
rbcL-accD_p2	rbcL-accD	2	ACTACAGTTCGGTGGAGGAA	GCCGGGGTGATATTTGTCAT	20	20	50.0	50.0	58.0	58.0	0.0	2.2	0.024114	56873	57326	911-911	0.500000	59.8
rbcL-accD_p3	rbcL-accD	3	GGAGTCTTGAACTAGCTGCC	GACATTGCCGGGGTGATATT	20	20	55.0	50.0	58.0	58.0	0.0	2.2	0.035881	56873	57326	762-762	0.500000	59.7
rbcL-accD_p4	rbcL-accD	4	GGAGTCTTGAACTAGCTGCC	GCCGGGGTGATATTTGTCAT	20	20	55.0	50.0	58.0	58.0	0.0	2.2	0.035881	56873	57326	756-756	0.500000	59.7
rbcL-accD_p5	rbcL-accD	5	TTGAATGCTACTGCGGGTAC	GCCGGGGTGATATTTGTCAT	20	20	50.0	50.0	58.0	58.0	0.0	2.2	0.037285	56873	57326	1390-1390	0.500000	59.7
accD_p1	accD	1	GGAGTCTTGAACTAGCTGCC	AAAGAAGCCATTGCCATTGC	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.120675	57327	59075	2690-2797	1.000000	85.4
accD_p2	accD	2	ACTACAGTTCGGTGGAGGAA	AAAGAAGCCATTGCCATTGC	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.108907	57327	59075	2845-2952	1.000000	85.3
accD_p3	accD	3	GGAGTCTTGAACTAGCTGCC	TTGCCATTGCCGGAAATACT	20	20	55.0	45.0	58.0	58.2	0.0	7.5	0.171131	57327	59075	2680-2787	1.000000	85.0
accD_p4	accD	4	ACTACAGTTCGGTGGAGGAA	TTGCCATTGCCGGAAATACT	20	20	50.0	45.0	58.0	58.2	0.0	7.5	0.159364	57327	59075	2835-2942	1.000000	84.9
accD_p5	accD	5	GCTCTGACCGAGATCTTTGG	AAAGAAGCCATTGCCATTGC	20	20	55.0	45.0	58.1	57.9	12.2	0.0	0.170291	57327	59075	2877-2984	1.000000	84.8
psaI-ycf4_p1	psaI-ycf4	1	TAGGCCTAGTATTTCCGGCA	TTGCATTTTTCCCTCCTGCT	20	20	50.0	45.0	57.9	58.0	8.9	0.0	0.105108	59505	60016	1127-1180	1.000000	87.6
psaI-ycf4_p2	psaI-ycf4	2	GCAATGGCAATGGCTTCTTT	CCAGGAAATTGTGGACCACA	20	20	45.0	50.0	57.9	58.0	8.6	23.7	0.109581	59505	60016	754-788	1.000000	87.1
psaI-ycf4_p3	psaI-ycf4	3	TAGGCCTAGTATTTCCGGCA	CCAGGAAATTGTGGACCACA	20	20	50.0	50.0	57.9	58.0	8.9	23.7	0.097142	59505	60016	771-805	1.000000	87.0
psaI-ycf4_p4	psaI-ycf4	4	TTTGAAGTTCCACGGCTTCA	TTGCATTTTTCCCTCCTGCT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.059571	59505	60016	1587-1587	1.000000	86.0
psaI-ycf4_p5	psaI-ycf4	5	TTTGAAGTTCCACGGCTTCA	CCAGGAAATTGTGGACCACA	20	20	45.0	50.0	58.0	58.0	0.0	23.7	0.051605	59505	60016	1212-1231	1.000000	85.2
ycf4_p1	ycf4	1	TAGGCCTAGTATTTCCGGCA	TTGCATTTTTCCCTCCTGCT	20	20	50.0	45.0	57.9	58.0	8.9	0.0	0.105108	60017	60538	1127-1180	1.000000	87.6
ycf4_p2	ycf4	2	AAAACAGGAGCAGATGCCAA	TTGCATTTTTCCCTCCTGCT	20	20	45.0	45.0	58.0	58.0	0.1	0.0	0.014362	60017	60538	652-652	0.500000	59.9
ycf4_p3	ycf4	3	AAAACAGGAGCAGATGCCAA	TGGCTCGACTGAATGTTCTG	20	20	45.0	50.0	58.0	57.9	0.1	0.0	0.084961	60017	60538	715-715	0.500000	59.3
ycf4_p4	ycf4	4	AAAACAGGAGCAGATGCCAA	ACAAGACAGAAATGGGCCAG	20	20	45.0	50.0	58.0	58.1	0.1	13.8	0.085211	60017	60538	1044-1044	0.500000	59.3
ycf4_p5	ycf4	5	AAAACAGGAGCAGATGCCAA	CAAGACAGAAATGGGCCAGT	20	20	45.0	50.0	58.0	58.1	0.1	13.8	0.085211	60017	60538	1043-1043	0.500000	59.3
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	63287	64285	1056-1059	1.000000	75.8
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	63287	64285	1058-1061	1.000000	75.8
petA-psbJ_p3	petA-psbJ	3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	63287	64285	1055-1058	1.000000	75.3
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	63287	64285	1057-1060	1.000000	75.3
petA-psbJ_p5	petA-psbJ	5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	57.7	57.5	5.4	0.0	0.775892	63287	64285	1055-1058	1.000000	74.9
rpl33-rps18_p1	rpl33-rps18	1	ATTCAACGTCTGCGAGACTC	AAAACACCAATCCGCGTTTG	20	20	50.0	45.0	58.0	58.2	12.4	4.9	0.169486	67976	69069	1552-2282	1.000000	84.4
rpl33-rps18_p2	rpl33-rps18	2	ATTCAACGTCTGCGAGACTC	CAAAACACCAATCCGCGTTT	20	20	50.0	45.0	58.0	58.2	12.4	4.9	0.169486	67976	69069	1553-2283	1.000000	84.4
rpl33-rps18_p3	rpl33-rps18	3	GATTCAACGTCTGCGAGACT	AAAACACCAATCCGCGTTTG	20	20	50.0	45.0	58.0	58.2	12.4	4.9	0.169486	67976	69069	1553-2283	1.000000	84.4
rpl33-rps18_p4	rpl33-rps18	4	GATTCAACGTCTGCGAGACT	CAAAACACCAATCCGCGTTT	20	20	50.0	45.0	58.0	58.2	12.4	4.9	0.169486	67976	69069	1554-2284	1.000000	84.4
rpl33-rps18_p5	rpl33-rps18	5	AAAGGAGGTTCATGGCCAAG	AAAACACCAATCCGCGTTTG	20	20	50.0	45.0	58.1	58.2	11.0	4.9	0.224591	67976	69069	1245-1965	1.000000	84.2
rps18_p1	rps18	1	AAAGGAGGTTCATGGCCAAG	GACCAGAACTAAACGCGGAT	20	20	50.0	50.0	58.1	58.0	11.0	12.5	0.077102	69070	69666	1792-2512	1.000000	85.1
rps18_p2	rps18	2	AAGAAGAAAGATGCGCGAGT	GACCAGAACTAAACGCGGAT	20	20	45.0	50.0	57.9	58.0	12.1	12.5	0.092025	69070	69666	1775-2495	1.000000	85.0
rps18_p3	rps18	3	AGGTGTCAACGGGAATTTCC	GACCAGAACTAAACGCGGAT	20	20	50.0	50.0	58.1	58.0	6.9	12.5	0.100333	69070	69666	1708-2428	1.000000	85.0
rps18_p4	rps18	4	AAAGGAGGTTCATGGCCAAG	TATCAAGTTTTCGAGGGGCG	20	20	50.0	50.0	58.1	58.0	11.0	0.0	0.089674	69070	69666	2453-2453	0.500000	59.3
rps18_p5	rps18	5	AAGAAGAAAGATGCGCGAGT	TATCAAGTTTTCGAGGGGCG	20	20	45.0	50.0	57.9	58.0	12.1	0.0	0.104596	69070	69666	2436-2436	0.500000	59.2
rps12-clpP_p1	rps12-clpP	1	TCATTCATACCGGAAAGCGG	CGCGACTCCATCATAGATGG	20	20	50.0	55.0	58.1	58.0	9.5	17.8	0.063832	71181	71364	524-583	1.000000	82.5
rps12-clpP_p2	rps12-clpP	2	TCTTTGGCTCTACCCCTGAA	TCTGTTCGTTTCACACAGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.056001	71181	71364	1012-1069	1.000000	81.9
rps12-clpP_p3	rps12-clpP	3	TCTTTGGCTCTACCCCTGAA	TGTTCGTTTCACACAGCAGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.056001	71181	71364	1010-1067	1.000000	81.9
rps12-clpP_p4	rps12-clpP	4	TCTTTGGCTCTACCCCTGAA	CGCGACTCCATCATAGATGG	20	20	50.0	55.0	58.0	58.0	0.0	17.8	0.050800	71181	71364	883-940	1.000000	81.6
rps12-clpP_p5	rps12-clpP	5	TCTTTGGCTCTACCCCTGAA	TAGAAAGCAACGGAGCCATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.063039	71181	71364	1240-1297	1.000000	81.6
clpP_p1	clpP	1	TCATTCATACCGGAAAGCGG	ATACCAAGGCAAACCCATGG	20	20	50.0	50.0	58.1	58.1	9.5	4.1	0.187227	71365	73406	2879-2992	1.000000	82.6
clpP_p2	clpP	2	AGTCGCACATACACCCTAGT	ATACCAAGGCAAACCCATGG	20	20	50.0	50.0	58.2	58.1	0.0	4.1	0.286679	71365	73406	2820-2933	1.000000	81.9
clpP_p3	clpP	3	TCGAATTGGCTGTCTTGCAT	TTCGTTACAGTCCCTCCTGT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.180280	71365	73406	2988-2988	0.500000	58.6
clpP_p4	clpP	4	AGTCGCACATACACCCTAGT	ACGAACATACCCTGTCTCCA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.218327	71365	73406	2987-2987	0.500000	58.3
clpP_p5	clpP	5	TCGAATTGGCTGTCTTGCAT	ACGAACATACCCTGTCTCCA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.241888	71365	73406	2913-2913	0.500000	58.1
rpoA_p1	rpoA	1	TTGGTTGGGTATTGGTGCAA	CGCCTTTGTACGGGATGTAA	20	20	45.0	50.0	57.9	58.0	0.0	3.9	0.101041	79315	80445	1427-1562	1.000000	90.6
rpoA_p2	rpoA	2	TTTGGTTGGGTATTGGTGCA	CGCCTTTGTACGGGATGTAA	20	20	45.0	50.0	57.9	58.0	0.0	3.9	0.101041	79315	80445	1428-1563	1.000000	90.6
rpoA_p3	rpoA	3	CAAAATCCATTTCGCCGTCC	CGCCTTTGTACGGGATGTAA	20	20	50.0	50.0	58.1	58.0	0.0	3.9	0.104872	79315	80445	1489-1624	1.000000	90.3
rpoA_p4	rpoA	4	CCAAAATCCATTTCGCCGTC	CGCCTTTGTACGGGATGTAA	20	20	50.0	50.0	58.1	58.0	0.0	3.9	0.104872	79315	80445	1490-1625	1.000000	90.3
rpoA_p5	rpoA	5	TTGGTTGGGTATTGGTGCAA	AGGGTACGAGAAAAGGGACA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.107912	79315	80445	1590-1725	1.000000	89.8
rpoA-rps11_p1	rpoA-rps11	1	AGTGGAGCCAGAATAAAGCG	CGCCTTTGTACGGGATGTAA	20	20	50.0	50.0	58.0	58.0	0.0	3.9	0.042775	80446	80516	251-251	1.000000	90.8
rpoA-rps11_p2	rpoA-rps11	2	AGTGGAGCCAGAATAAAGCG	GATTCGCCTTTGTACGGGAT	20	20	50.0	50.0	58.0	58.1	0.0	3.9	0.080373	80446	80516	255-255	1.000000	90.5
rpoA-rps11_p3	rpoA-rps11	3	AGTGGAGCCAGAATAAAGCG	AGGGTACGAGAAAAGGGACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049646	80446	80516	414-414	1.000000	87.7
rpoA-rps11_p4	rpoA-rps11	4	AGTGGAGCCAGAATAAAGCG	CCGAGAACTGGTTCACGTAG	20	20	50.0	55.0	58.0	58.0	0.0	23.4	0.051297	80446	80516	631-631	1.000000	85.2
rpoA-rps11_p5	rpoA-rps11	5	AGTGGAGCCAGAATAAAGCG	CGAGAACTGGTTCACGTAGG	20	20	50.0	55.0	58.0	58.0	0.0	19.5	0.051297	80446	80516	630-630	1.000000	85.2
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	AGGATTCGAACCTACGACCA	ACGACAACGACCGAATTGAT	20	20	50.0	45.0	58.2	57.9	15.0	0.0	0.231179	109427	110252	740-965	1.000000	84.4
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	CCCCAAGTAGGATTCGAACC	ACGACAACGACCGAATTGAT	20	20	55.0	45.0	57.7	57.9	0.0	0.0	0.401747	109427	110252	748-973	1.000000	83.0
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	AGGATTCGAACCTACGACCA	TCCATAATAGAGCCCGACGA	20	20	50.0	50.0	58.2	57.7	15.0	0.0	0.438171	109427	110252	756-981	1.000000	82.7
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	AGGATTCGAACCTACGACCA	CCATAATAGAGCCCGACGAC	20	20	50.0	55.0	58.2	57.7	15.0	0.0	0.505929	109427	110252	755-980	1.000000	82.2
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	CCCCAAGTAGGATTCGAACC	TCCATAATAGAGCCCGACGA	20	20	55.0	50.0	57.7	57.7	0.0	0.0	0.608739	109427	110252	764-989	1.000000	81.3
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	ACGACAACGACCGAATTGAT	AGGATTCGAACCTACGACCA	20	20	45.0	50.0	57.9	58.2	0.0	15.0	0.231179	135484	136309	740-965	1.000000	84.4
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	ACGACAACGACCGAATTGAT	CCCCAAGTAGGATTCGAACC	20	20	45.0	55.0	57.9	57.7	0.0	0.0	0.401747	135484	136309	748-973	1.000000	83.0
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	TCCATAATAGAGCCCGACGA	AGGATTCGAACCTACGACCA	20	20	50.0	50.0	57.7	58.2	0.0	15.0	0.438171	135484	136309	756-981	1.000000	82.7
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	CCATAATAGAGCCCGACGAC	AGGATTCGAACCTACGACCA	20	20	55.0	50.0	57.7	58.2	0.0	15.0	0.505929	135484	136309	755-980	1.000000	82.2
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	TCCATAATAGAGCCCGACGA	CCCCAAGTAGGATTCGAACC	20	20	50.0	55.0	57.7	57.7	0.0	0.0	0.608739	135484	136309	764-989	1.000000	81.3