Hotspot

trnL-UAG-ndhF

SSC · 125411-127270 · 1860 bp

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Pi 0.0165
MarkerSeek score 61.90
Primer available yes
Label trnL-UAG-ndhF

Feature Metadata

label_name
trnL-UAG-ndhF
feature_type
igs
region
SSC
length_bp
1860
variable_sites
72
pi
0.0165
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ndhF_p1 TCCTAAGAGCAGCGTGTCTA CTGGAAGTGGAATCAAAGGT 50.0 45.0 57.9 55.2 1951-2325 bp 1.0 60.1
trnL-UAG-ndhF_p2 AGCAGCGTGTCTACCAATTT CTGGAAGTGGAATCAAAGGT 45.0 45.0 57.8 55.2 1944-2318 bp 1.0 59.7
trnL-UAG-ndhF_p3 CCTAAGAGCAGCGTGTCTAC CTGGAAGTGGAATCAAAGGT 55.0 45.0 57.8 55.2 1950-2324 bp 1.0 59.6
trnL-UAG-ndhF_p4 CTAAGAGCAGCGTGTCTACC CTGGAAGTGGAATCAAAGGT 55.0 45.0 57.8 55.2 1949-2323 bp 1.0 59.6
trnL-UAG-ndhF_p5 GAGCAGCGTGTCTACCAATT CTGGAAGTGGAATCAAAGGT 50.0 45.0 58.3 55.2 1945-2319 bp 1.0 58.9