Hotspot

ycf4-cemA

LSC · 61649-62160 · 512 bp

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Pi 0.0189

MarkerSeek score 58.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 23
pi: 0.0189
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	AATCGTCGCATCTTCCTACG	GGCGAATGGAATCCGATACA	50.0	50.0	57.9	57.8	1227-1231 bp	1.0	80.0
ycf4-cemA_p2	CGTCGCATCTTCCTACGATT	GGCGAATGGAATCCGATACA	50.0	50.0	57.9	57.8	1224-1228 bp	1.0	80.0
ycf4-cemA_p3	TGAATTGGCGATCAGAACGT	GGCGAATGGAATCCGATACA	45.0	50.0	57.9	57.8	1562-1566 bp	1.0	79.8
ycf4-cemA_p4	AAAAAGCTGCTGAATTGGCC	GGCGAATGGAATCCGATACA	45.0	50.0	57.8	57.8	1061-1065 bp	1.0	79.8
ycf4-cemA_p5	TTTGCTGGGCCTTTATCCTC	GGCGAATGGAATCCGATACA	50.0	50.0	57.9	57.8	1499-1503 bp	1.0	79.5