Hotspot

ndhC-trnM-CAU

LSC · 52365-53811 · 1447 bp

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Pi 0.0094

MarkerSeek score 57.50

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1447
variable_sites: 32
pi: 0.0094
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	CTGGTCCTTTGCTAATCGGG	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.3	58.4	1605-1606 bp	1.0	75.7
ndhC-trnM-CAU_p2	CCTTTGCTAATCGGGGCTAA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.7	58.4	1600-1601 bp	1.0	75.6
ndhC-trnM-CAU_p3	TCCTTTGCTAATCGGGGCTA	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.5	58.4	1601-1602 bp	1.0	74.4
ndhC-trnM-CAU_p4	CTGGTCCTTTGCTAATCGGG	CCGCCGTATGAAAGCAATAC	55.0	50.0	58.3	57.3	1604-1605 bp	1.0	73.7
ndhC-trnM-CAU_p5	CCTTTGCTAATCGGGGCTAA	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.7	57.3	1599-1600 bp	1.0	73.6