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psaA-ycf3

LSC · 42728-43565 · 838 bp

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Pi 0.00538

MarkerSeek score 59.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 11
pi: 0.00538
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCACTCCTCAAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.7	58.0	994-1044 bp	1.0	80.1
psaA-ycf3_p2	GCCCACTCCTCAAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.7	58.0	976-1026 bp	1.0	80.1
psaA-ycf3_p3	GCCCACTCCTCAAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.7	57.8	923-973 bp	1.0	79.3
psaA-ycf3_p4	GCCCACTCCTCAAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.7	58.1	996-1046 bp	1.0	79.2
psaA-ycf3_p5	GCCCACTCCTCAAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.7	58.2	927-977 bp	1.0	79.0