Hotspot

trnD-GUC-trnY-GUA

LSC · 30739-31159 · 421 bp

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Pi 0.0162
MarkerSeek score 57.10
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
421
variable_sites
16
pi
0.0162
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA GCAAGATGTCTACGCTGGTT 50.0 50.0 58.5 58.3 489 bp 1.0 76.6
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GTTGGCAAGATGTCTACGCT 50.0 50.0 58.5 58.3 493 bp 1.0 76.6
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA TTGGCAAGATGTCTACGCTG 50.0 50.0 58.5 58.3 492 bp 1.0 76.6
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 512 bp 1.0 75.9
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 511 bp 1.0 75.7