Hotspot

rpoB-trnC-GCA

LSC · 26910-28141 · 1232 bp

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Pi 0.00868

MarkerSeek score 57.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1232
variable_sites: 26
pi: 0.00868
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTTCATCCGGGAG	CCGGATTTGAACTGGGGAAT	55.0	50.0	58.0	57.9	1307-1318 bp	1.0	80.9
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.0	57.9	1380-1391 bp	1.0	80.8
rpoB-trnC-GCA_p3	CTCATTTTCATCCGGGAGCA	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.0	57.9	1305-1316 bp	1.0	80.8
rpoB-trnC-GCA_p4	CCCTCATTTTCATCCGGGAG	ATTTGCAATCCTCCGCCTTA	55.0	45.0	58.0	57.6	1282-1293 bp	1.0	78.6
rpoB-trnC-GCA_p5	CGTCAAGCCCTGATCAATGA	ATTTGCAATCCTCCGCCTTA	50.0	45.0	58.0	57.6	1355-1366 bp	1.0	78.5