Hotspot

ycf1

IRb · 114726-115691 · 966 bp

Back to Talipariti

Pi 0

MarkerSeek score 38.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 966
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	ATATGTAGGGGGAAGTCGCA	55.0	50.0	58.0	57.9	1660-1711 bp	1.0	79.4
ycf1_p2	GAGCTACTGAGGAACAACGG	TATGTAGGGGGAAGTCGCAT	55.0	50.0	58.0	57.9	1659-1710 bp	1.0	79.4
ycf1_p3	GGGTTACCTGCTCTTAACGG	ATATGTAGGGGGAAGTCGCA	55.0	50.0	58.0	57.9	1814-1865 bp	1.0	79.2
ycf1_p4	GGGTTACCTGCTCTTAACGG	TATGTAGGGGGAAGTCGCAT	55.0	50.0	58.0	57.9	1813-1864 bp	1.0	79.2
ycf1_p5	ACGGTCAAAGCGAACTCTTT	TATGTAGGGGGAAGTCGCAT	45.0	50.0	58.1	57.9	1797-1848 bp	1.0	79.0