Hotspot

clpP

LSC · 74537-76721 · 2185 bp

Back to Talipariti

Pi 0.00183

MarkerSeek score 58.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2185
variable_sites: 4
pi: 0.00183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.8	57.7	2265-2266 bp	1.0	60.8
clpP_p2	CCCCTTAGGCTTACGTTAAGA	ACGCTTCCACATCAAAGTGA	47.6	45.0	56.8	57.7	2328-2329 bp	1.0	60.7
clpP_p3	GGATTGGTGTTAAATCGTTGCT	ACGCTTCCACATCAAAGTGA	40.9	45.0	57.6	57.7	2264-2265 bp	1.0	58.6
clpP_p4	CCCCTTAGGCTTACGTTAAGAA	ACGCTTCCACATCAAAGTGA	45.5	45.0	57.5	57.7	2328-2329 bp	1.0	58.3
clpP_p5	CCCCTTAGGCTTACGTTAAG	ACGCTTCCACATCAAAGTGA	50.0	45.0	55.3	57.7	2328-2329 bp	1.0	56.1