Hotspot

trnN_guu-trnR_acg

IRa · 136608-137261 · 654 bp

Back to Talipariti

Pi 0.00153

MarkerSeek score 59.60

Primer available yes

Label trnN_guu-trnR_acg

Feature Metadata

label_name: trnN_guu-trnR_acg
feature_type: igs
region: IRa
length_bp: 654
variable_sites: 1
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN_guu-trnR_acg_p1	CCGTTGTTCCTCAGTAGCTC	AACAGATCCAGTGGAGACGA	55.0	50.0	58.0	58.1	831 bp	1.0	79.5
trnN_guu-trnR_acg_p2	TGGGTTGAGAACGGGAATTG	AACAGATCCAGTGGAGACGA	50.0	50.0	58.1	58.1	872 bp	1.0	78.7
trnN_guu-trnR_acg_p3	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	795 bp	1.0	78.4
trnN_guu-trnR_acg_p4	GCTGTTAACTGACTGGTCGT	AACAGATCCAGTGGAGACGA	50.0	50.0	57.8	58.1	795 bp	1.0	78.3
trnN_guu-trnR_acg_p5	TGGTCGTAGGTTCGAATCCT	AACAGATCCAGTGGAGACGA	50.0	50.0	58.2	58.1	782 bp	1.0	78.2