Hotspot

rpl32-trnL_uag

SSC · 119528-120691 · 1164 bp

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Pi 0.00172

MarkerSeek score 50.20

Primer available yes

Label rpl32-trnL_uag

Feature Metadata

label_name: rpl32-trnL_uag
feature_type: igs
region: SSC
length_bp: 1164
variable_sites: 2
pi: 0.00172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_uag_p1	CGGCGTTGAAAGCTTTTTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1279 bp	1.0	78.7
rpl32-trnL_uag_p2	CGGCGTTGAAAGCTTTTTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1280 bp	1.0	78.7
rpl32-trnL_uag_p3	CGGCGTTGAAAGCTTTTTCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	1289 bp	1.0	77.8
rpl32-trnL_uag_p4	AAGAAAGGATATTGGGCGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	1295 bp	1.0	77.6
rpl32-trnL_uag_p5	AAGAAAGGATATTGGGCGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	1296 bp	1.0	77.6