Hotspot

trnR_acg-trnN_guu

IRb · 113686-114339 · 654 bp

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Pi 0.00153

MarkerSeek score 59.60

Primer available yes

Label trnR_acg-trnN_guu

Feature Metadata

label_name: trnR_acg-trnN_guu
feature_type: igs
region: IRb
length_bp: 654
variable_sites: 1
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR_acg-trnN_guu_p1	AACAGATCCAGTGGAGACGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.1	58.0	831 bp	1.0	79.5
trnR_acg-trnN_guu_p2	AACAGATCCAGTGGAGACGA	TGGGTTGAGAACGGGAATTG	50.0	50.0	58.1	58.1	872 bp	1.0	78.7
trnR_acg-trnN_guu_p3	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	795 bp	1.0	78.4
trnR_acg-trnN_guu_p4	AACAGATCCAGTGGAGACGA	GCTGTTAACTGACTGGTCGT	50.0	50.0	58.1	57.8	795 bp	1.0	78.3
trnR_acg-trnN_guu_p5	AACAGATCCAGTGGAGACGA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	58.1	58.2	782 bp	1.0	78.2