Hotspot

petA-psbJ

LSC · 67261-68364 · 1104 bp

Back to Talipariti

Pi 0.0174

MarkerSeek score 62.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1104
variable_sites: 19
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.8	57.6	1224-1232 bp	1.0	77.2
petA-psbJ_p2	TGAGAAGGTTCAAGTGTCCG	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.5	57.6	1156-1164 bp	1.0	74.4
petA-psbJ_p3	GAGAAGGTTCAAGTGTCCGA	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.2	57.6	1155-1163 bp	1.0	72.1
petA-psbJ_p4	GCCTTTTGTTCTTCTTGGCA	ATTCTGGATTGGGTTCGTCC	45.0	50.0	57.1	57.6	1223-1231 bp	1.0	71.4
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	CTGGTATTCCTGTGATCGGT	50.0	50.0	57.8	56.7	1272-1280 bp	1.0	70.3