Hotspot

trnT_ugu-trnL_uaa

LSC · 49553-50304 · 752 bp

Back to Talipariti

Pi 0.00133

MarkerSeek score 59.50

Primer available yes

Label trnT_ugu-trnL_uaa

Feature Metadata

label_name: trnT_ugu-trnL_uaa
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 1
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT_ugu-trnL_uaa_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	813 bp	1.0	77.0
trnT_ugu-trnL_uaa_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	845 bp	1.0	76.2
trnT_ugu-trnL_uaa_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	807 bp	1.0	75.8
trnT_ugu-trnL_uaa_p4	CAAATAGGGAAAGCCGGCTA	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.7	58.2	867 bp	1.0	75.4
trnT_ugu-trnL_uaa_p5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	845 bp	1.0	75.0