Hotspot

rps4-trnT_ugu

LSC · 48903-49479 · 577 bp

Back to Talipariti

Pi 0.00351

MarkerSeek score 61.50

Primer available yes

Label rps4-trnT_ugu

Feature Metadata

label_name: rps4-trnT_ugu
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 2
pi: 0.00351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT_ugu_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	730-737 bp	1.0	79.7
rps4-trnT_ugu_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	734-741 bp	1.0	79.4
rps4-trnT_ugu_p3	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	698-705 bp	1.0	78.9
rps4-trnT_ugu_p4	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	702-709 bp	1.0	78.6
rps4-trnT_ugu_p5	TAAGATCGCTTCCGGCTCTA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.0	57.5	738-745 bp	1.0	76.8