Hotspot

petN-psbM

LSC · 30112-31274 · 1163 bp

Back to Talipariti

Pi 0.00344

MarkerSeek score 60.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 4
pi: 0.00344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACAGCGCTGTTTATTCT	45.0	42.9	58.0	58.0	1318 bp	1.0	72.0
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACAGCGCTGTTTATTCT	50.0	42.9	57.8	58.0	1324 bp	1.0	70.6
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	TGCTACAGCGCTGTTTATTC	45.0	45.0	58.0	56.5	1318 bp	1.0	68.5
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	TGCTACAGCGCTGTTTATTC	50.0	45.0	57.8	56.5	1324 bp	1.0	67.1
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	CATTTATTGCTACAGCGCTGT	45.0	42.9	58.0	57.3	1325 bp	1.0	66.8