Hotspot

rps16-trnQ_uug

LSC · 6625-7191 · 567 bp

Back to Talipariti

Pi 0.00178

MarkerSeek score 60.10

Primer available yes

Label rps16-trnQ_uug

Feature Metadata

label_name: rps16-trnQ_uug
feature_type: igs
region: LSC
length_bp: 567
variable_sites: 1
pi: 0.00178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_uug_p1	AGTGGTGGGTTTTGACATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	673-679 bp	1.0	80.2
rps16-trnQ_uug_p2	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	652-658 bp	1.0	80.0
rps16-trnQ_uug_p3	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	650-656 bp	1.0	80.0
rps16-trnQ_uug_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	634-640 bp	1.0	79.2
rps16-trnQ_uug_p5	TTTTGACATCCACAGCCGAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	664-670 bp	1.0	78.6