Hotspot

trnK_uuu-rps16

LSC · 4579-5488 · 910 bp

Back to Talipariti

Pi 0.00115

MarkerSeek score 63.30

Primer available yes

Label trnK_uuu-rps16

Feature Metadata

label_name: trnK_uuu-rps16
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 1
pi: 0.00115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK_uuu-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	1001-1038 bp	1.0	81.5
trnK_uuu-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.2	58.0	1000-1037 bp	1.0	81.5
trnK_uuu-rps16_p3	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.5	58.0	987-1024 bp	1.0	79.2
trnK_uuu-rps16_p4	AAAGCCGAGTACTCTACCGT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	961-998 bp	1.0	78.8
trnK_uuu-rps16_p5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	50.0	50.0	58.2	58.4	960-997 bp	1.0	78.8