Hotspot

psbA-trnK_uuu

LSC · 1654-1946 · 293 bp

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Pi 0

MarkerSeek score 32.20

Primer available yes

Label psbA-trnK_uuu

Feature Metadata

label_name: psbA-trnK_uuu
feature_type: igs
region: LSC
length_bp: 293
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK_uuu_p1	TAAAGACGGTTTTCGGTGCT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.8	58.6	421 bp	1.0	73.2
psbA-trnK_uuu_p2	AACACCAAACCATCCGATGT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	440 bp	1.0	72.8
psbA-trnK_uuu_p3	GTAAAGACGGTTTTCGGTGC	ACTCCATCCGACTAGTTCCG	50.0	55.0	57.7	58.6	422 bp	1.0	72.7
psbA-trnK_uuu_p4	TAAAGACGGTTTTCGGTGCT	ACTAGTTCCGGGTTCGAGTC	45.0	55.0	57.8	58.8	411 bp	1.0	71.4
psbA-trnK_uuu_p5	TAAAGACGGTTTTCGGTGCT	GACTAGTTCCGGGTTCGAGT	45.0	55.0	57.8	58.8	412 bp	1.0	71.4