primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK_uuu_p1	psbA-trnK_uuu	1	TAAAGACGGTTTTCGGTGCT	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	57.8	58.6	0.0	0.0	0.853037	1654	1946	421-421	1.000000	73.2
psbA-trnK_uuu_p2	psbA-trnK_uuu	2	AACACCAAACCATCCGATGT	ACTCCATCCGACTAGTTCCG	20	20	45.0	55.0	57.7	58.6	7.6	0.0	0.895799	1654	1946	440-440	1.000000	72.8
psbA-trnK_uuu_p3	psbA-trnK_uuu	3	GTAAAGACGGTTTTCGGTGC	ACTCCATCCGACTAGTTCCG	20	20	50.0	55.0	57.7	58.6	0.0	0.0	0.914686	1654	1946	422-422	1.000000	72.7
psbA-trnK_uuu_p4	psbA-trnK_uuu	4	TAAAGACGGTTTTCGGTGCT	ACTAGTTCCGGGTTCGAGTC	20	20	45.0	55.0	57.8	58.8	0.0	0.0	1.071427	1654	1946	411-411	1.000000	71.4
psbA-trnK_uuu_p5	psbA-trnK_uuu	5	TAAAGACGGTTTTCGGTGCT	GACTAGTTCCGGGTTCGAGT	20	20	45.0	55.0	57.8	58.8	0.0	0.0	1.071427	1654	1946	412-412	1.000000	71.4
trnK_uuu-rps16_p1	trnK_uuu-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4579	5488	1001-1038	1.000000	81.5
trnK_uuu-rps16_p2	trnK_uuu-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	58.2	58.0	20.3	17.2	0.181537	4579	5488	1000-1037	1.000000	81.5
trnK_uuu-rps16_p3	trnK_uuu-rps16	3	TACCGTTGAGTTAGCAACCC	AAAAGGAGCTCAACCCACAG	20	20	50.0	50.0	57.5	58.0	0.0	17.2	0.473875	4579	5488	987-1024	1.000000	79.2
trnK_uuu-rps16_p4	trnK_uuu-rps16	4	AAAGCCGAGTACTCTACCGT	AAGAAGGCGGGGGTTTTTAC	20	20	50.0	50.0	58.2	58.4	20.3	0.0	0.544084	4579	5488	961-998	1.000000	78.8
trnK_uuu-rps16_p5	trnK_uuu-rps16	5	AAGCCGAGTACTCTACCGTT	AAGAAGGCGGGGGTTTTTAC	20	20	50.0	50.0	58.2	58.4	20.3	0.0	0.544084	4579	5488	960-997	1.000000	78.8
rps16-trnQ_uug_p1	rps16-trnQ_uug	1	AGTGGTGGGTTTTGACATCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.080725	6625	7191	673-679	1.000000	80.2
rps16-trnQ_uug_p2	rps16-trnQ_uug	2	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.104521	6625	7191	652-658	1.000000	80.0
rps16-trnQ_uug_p3	rps16-trnQ_uug	3	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.105939	6625	7191	650-656	1.000000	80.0
rps16-trnQ_uug_p4	rps16-trnQ_uug	4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6625	7191	634-640	1.000000	79.2
rps16-trnQ_uug_p5	rps16-trnQ_uug	5	TTTTGACATCCACAGCCGAT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.274928	6625	7191	664-670	1.000000	78.6
trnS_gcu-trnG_ucc_p1	trnS_gcu-trnG_ucc	1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.424641	8546	9399	903-969	1.000000	80.6
trnS_gcu-trnG_ucc_p2	trnS_gcu-trnG_ucc	2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.426029	8546	9399	902-968	1.000000	80.6
trnS_gcu-trnG_ucc_p3	trnS_gcu-trnG_ucc	3	CGCACAACGGATTAGCAATC	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.511210	8546	9399	918-984	1.000000	79.9
trnS_gcu-trnG_ucc_p4	trnS_gcu-trnG_ucc	4	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACCGAGGGACC	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.571407	8546	9399	957-1023	1.000000	79.4
trnS_gcu-trnG_ucc_p5	trnS_gcu-trnG_ucc	5	TCGCACAACGGATTAGCAAT	ATGAATCAAACCGAGGGACC	20	20	45.0	50.0	58.3	57.6	0.0	0.0	0.687118	8546	9399	919-985	1.000000	78.5
atpF-atpH_p1	atpF-atpH	1	CAGTGACCCAAGGAAACGAA	TGGTTGTAGCATTGGCACTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044251	13692	14303	694-697	1.000000	80.1
atpF-atpH_p2	atpF-atpH	2	CAGTGACCCAAGGAAACGAA	CTGGTTGTAGCATTGGCACT	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.500159	13692	14303	695-698	1.000000	76.5
atpF-atpH_p3	atpF-atpH	3	CAGTGACCCAAGGAAACGAA	ATGGGCTGGTTGTAGCATTG	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.560653	13692	14303	700-703	1.000000	76.0
atpF-atpH_p4	atpF-atpH	4	AGTGACCCAAGGAAACGAAA	TGGTTGTAGCATTGGCACTT	20	20	45.0	45.0	57.3	58.0	0.0	0.0	0.727469	13692	14303	693-696	1.000000	74.6
atpF-atpH_p5	atpF-atpH	5	CAGTGACCCAAGGAAACGAA	CCGAGGCAGAGGGAAAAATA	20	20	50.0	50.0	58.0	57.3	0.0	0.0	0.748302	13692	14303	769-772	1.000000	74.4
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACAGCGCTGTTTATTCT	20	21	45.0	42.9	58.0	58.0	0.0	44.3	1.033419	30112	31274	1318-1318	1.000000	72.0
petN-psbM_p2	petN-psbM	2	AGTAAGTCTTGCTTGGGCTG	TGCTACAGCGCTGTTTATTCT	20	21	50.0	42.9	57.8	58.0	0.0	44.3	1.218503	30112	31274	1324-1324	1.000000	70.6
petN-psbM_p3	petN-psbM	3	TCTTGCTTGGGCTGCTTTAA	TGCTACAGCGCTGTTTATTC	20	20	45.0	45.0	58.0	56.5	0.0	44.3	1.471392	30112	31274	1318-1318	1.000000	68.5
petN-psbM_p4	petN-psbM	4	AGTAAGTCTTGCTTGGGCTG	TGCTACAGCGCTGTTTATTC	20	20	50.0	45.0	57.8	56.5	0.0	44.3	1.656476	30112	31274	1324-1324	1.000000	67.1
petN-psbM_p5	petN-psbM	5	TCTTGCTTGGGCTGCTTTAA	CATTTATTGCTACAGCGCTGT	20	21	45.0	42.9	58.0	57.3	0.0	41.5	1.686686	30112	31274	1325-1325	1.000000	66.8
rps4-trnT_ugu_p1	rps4-trnT_ugu	1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.155684	48903	49479	730-737	1.000000	79.7
rps4-trnT_ugu_p2	rps4-trnT_ugu	2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.196808	48903	49479	734-741	1.000000	79.4
rps4-trnT_ugu_p3	rps4-trnT_ugu	3	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.263437	48903	49479	698-705	1.000000	78.9
rps4-trnT_ugu_p4	rps4-trnT_ugu	4	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.304561	48903	49479	702-709	1.000000	78.6
rps4-trnT_ugu_p5	rps4-trnT_ugu	5	TAAGATCGCTTCCGGCTCTA	CCGCTTAGCTCAGAGGTTAG	20	20	50.0	55.0	58.0	57.5	0.0	7.6	0.523453	48903	49479	738-745	1.000000	76.8
trnT_ugu-trnL_uaa_p1	trnT_ugu-trnL_uaa	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	49553	50304	813-813	1.000000	77.0
trnT_ugu-trnL_uaa_p2	trnT_ugu-trnL_uaa	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	49553	50304	845-845	1.000000	76.2
trnT_ugu-trnL_uaa_p3	trnT_ugu-trnL_uaa	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	49553	50304	807-807	1.000000	75.8
trnT_ugu-trnL_uaa_p4	trnT_ugu-trnL_uaa	4	CAAATAGGGAAAGCCGGCTA	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.7	58.2	33.1	10.2	0.590295	49553	50304	867-867	1.000000	75.4
trnT_ugu-trnL_uaa_p5	trnT_ugu-trnL_uaa	5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	49553	50304	845-845	1.000000	75.0
atpB-rbcL_p1	atpB-rbcL	1	TTTCAAGCGTGGAAACTCCA	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.164621	58022	59147	1194-1204	1.000000	80.0
atpB-rbcL_p2	atpB-rbcL	2	TTTCAAGCGTGGAAACTCCA	AACACCGGCTTTGAATCCAA	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.347055	58022	59147	1237-1247	1.000000	78.4
atpB-rbcL_p3	atpB-rbcL	3	TTTCAAGCGTGGAAACTCCA	TGGTGACATAAGTCCCTCCC	20	20	45.0	55.0	58.0	58.4	0.0	0.0	0.465502	58022	59147	1195-1205	1.000000	77.5
atpB-rbcL_p4	atpB-rbcL	4	TTTCAAGCGTGGAAACTCCA	TAACACCGGCTTTGAATCCA	20	20	45.0	45.0	58.0	57.4	0.0	0.0	0.615976	58022	59147	1238-1248	1.000000	76.3
atpB-rbcL_p5	atpB-rbcL	5	TTTCAAGCGTGGAAACTCCA	GTGGTGACATAAGTCCCTCC	20	20	45.0	55.0	58.0	57.3	0.0	0.0	0.738828	58022	59147	1196-1206	1.000000	75.4
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTGGC	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.574912	67261	68364	1224-1232	1.000000	77.2
petA-psbJ_p2	petA-psbJ	2	TGAGAAGGTTCAAGTGTCCG	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	57.5	57.6	0.0	0.0	0.946362	67261	68364	1156-1164	1.000000	74.4
petA-psbJ_p3	petA-psbJ	3	GAGAAGGTTCAAGTGTCCGA	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	57.2	57.6	0.0	0.0	1.234338	67261	68364	1155-1163	1.000000	72.1
petA-psbJ_p4	petA-psbJ	4	GCCTTTTGTTCTTCTTGGCA	ATTCTGGATTGGGTTCGTCC	20	20	45.0	50.0	57.1	57.6	0.0	0.0	1.308892	67261	68364	1223-1231	1.000000	71.4
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTGGC	CTGGTATTCCTGTGATCGGT	20	20	50.0	50.0	57.8	56.7	0.0	0.0	1.434514	67261	68364	1272-1280	1.000000	70.3
clpP_p1	clpP	1	TGGATTGGTGTTAAATCGTTGC	ACGCTTCCACATCAAAGTGA	22	20	40.9	45.0	57.8	57.7	0.0	3.0	2.422099	74537	76721	2265-2266	1.000000	60.8
clpP_p2	clpP	2	CCCCTTAGGCTTACGTTAAGA	ACGCTTCCACATCAAAGTGA	21	20	47.6	45.0	56.8	57.7	0.0	3.0	2.432800	74537	76721	2328-2329	1.000000	60.7
clpP_p3	clpP	3	GGATTGGTGTTAAATCGTTGCT	ACGCTTCCACATCAAAGTGA	22	20	40.9	45.0	57.6	57.7	0.0	3.0	2.689259	74537	76721	2264-2265	1.000000	58.6
clpP_p4	clpP	4	CCCCTTAGGCTTACGTTAAGAA	ACGCTTCCACATCAAAGTGA	22	20	45.5	45.0	57.5	57.7	0.0	3.0	2.731173	74537	76721	2328-2329	1.000000	58.3
clpP_p5	clpP	5	CCCCTTAGGCTTACGTTAAG	ACGCTTCCACATCAAAGTGA	20	20	50.0	45.0	55.3	57.7	0.0	3.0	2.999433	74537	76721	2328-2329	1.000000	56.1
trnR_acg-trnN_guu_p1	trnR_acg-trnN_guu	1	AACAGATCCAGTGGAGACGA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.1	58.0	18.7	0.0	0.077600	113686	114339	831-831	1.000000	79.5
trnR_acg-trnN_guu_p2	trnR_acg-trnN_guu	2	AACAGATCCAGTGGAGACGA	TGGGTTGAGAACGGGAATTG	20	20	50.0	50.0	58.1	58.1	18.7	0.0	0.170982	113686	114339	872-872	1.000000	78.7
trnR_acg-trnN_guu_p3	trnR_acg-trnN_guu	3	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	113686	114339	795-795	1.000000	78.4
trnR_acg-trnN_guu_p4	trnR_acg-trnN_guu	4	AACAGATCCAGTGGAGACGA	GCTGTTAACTGACTGGTCGT	20	20	50.0	50.0	58.1	57.8	18.7	15.9	0.225666	113686	114339	795-795	1.000000	78.3
trnR_acg-trnN_guu_p5	trnR_acg-trnN_guu	5	AACAGATCCAGTGGAGACGA	TGGTCGTAGGTTCGAATCCT	20	20	50.0	50.0	58.1	58.2	18.7	18.9	0.231901	113686	114339	782-782	1.000000	78.2
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	ATATGTAGGGGGAAGTCGCA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.099441	114726	115691	1660-1711	1.000000	79.4
ycf1_p2	ycf1	2	GAGCTACTGAGGAACAACGG	TATGTAGGGGGAAGTCGCAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.099441	114726	115691	1659-1710	1.000000	79.4
ycf1_p3	ycf1	3	GGGTTACCTGCTCTTAACGG	ATATGTAGGGGGAAGTCGCA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.117058	114726	115691	1814-1865	1.000000	79.2
ycf1_p4	ycf1	4	GGGTTACCTGCTCTTAACGG	TATGTAGGGGGAAGTCGCAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.117058	114726	115691	1813-1864	1.000000	79.2
ycf1_p5	ycf1	5	ACGGTCAAAGCGAACTCTTT	TATGTAGGGGGAAGTCGCAT	20	20	45.0	50.0	58.1	57.9	5.5	0.0	0.147645	114726	115691	1797-1848	1.000000	79.0
ycf1-ndhF_p1	ycf1-ndhF	1	TTTTTCGGAGGAAAGGGTGG	ATATGTAGGGGGAAGTCGCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.107404	115692	115967	458-509	1.000000	79.8
ycf1-ndhF_p2	ycf1-ndhF	2	TTTTTCGGAGGAAAGGGTGG	TATGTAGGGGGAAGTCGCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.107404	115692	115967	457-508	1.000000	79.8
ycf1-ndhF_p3	ycf1-ndhF	3	GTGGATCCGAGCAAAATCGA	ATATGTAGGGGGAAGTCGCA	20	20	50.0	50.0	58.3	57.9	7.4	0.0	0.445123	115692	115967	442-493	1.000000	77.1
ycf1-ndhF_p4	ycf1-ndhF	4	GTGGATCCGAGCAAAATCGA	TATGTAGGGGGAAGTCGCAT	20	20	50.0	50.0	58.3	57.9	7.4	0.0	0.445123	115692	115967	441-492	1.000000	77.1
ycf1-ndhF_p5	ycf1-ndhF	5	TTTTTCGGAGGAAAGGGTGG	GGGAAGTCGCATCTCGTTTT	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.570615	115692	115967	449-500	1.000000	76.1
rpl32-trnL_uag_p1	rpl32-trnL_uag	1	CGGCGTTGAAAGCTTTTTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.173970	119528	120691	1279-1279	1.000000	78.7
rpl32-trnL_uag_p2	rpl32-trnL_uag	2	CGGCGTTGAAAGCTTTTTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.2	58.0	14.0	0.0	0.175341	119528	120691	1280-1280	1.000000	78.7
rpl32-trnL_uag_p3	rpl32-trnL_uag	3	CGGCGTTGAAAGCTTTTTCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	14.0	0.0	0.290417	119528	120691	1289-1289	1.000000	77.8
rpl32-trnL_uag_p4	rpl32-trnL_uag	4	AAGAAAGGATATTGGGCGGC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.315420	119528	120691	1295-1295	1.000000	77.6
rpl32-trnL_uag_p5	rpl32-trnL_uag	5	AAGAAAGGATATTGGGCGGC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.316790	119528	120691	1296-1296	1.000000	77.6
trnL_uag_p1	trnL_uag	1	TGAATTGACTTCAATCTCGACG	GAGGGTTTCTCGATTTTATGGA	22	22	40.9	40.9	56.8	55.9	4.9	0.0	7.283122	120692	120771	219-219	1.000000	40.4
trnL_uag_p2	trnL_uag	2	TGAATTGACTTCAATCTCGACGA	GAGGGTTTCTCGATTTTATGGA	23	22	39.1	40.9	58.2	55.9	4.9	0.0	7.310021	120692	120771	219-219	1.000000	40.4
trnL_uag_p3	trnL_uag	3	TGAATTGACTTCAATCTCGACG	GGGTTTCTCGATTTTATGGAATGG	22	24	40.9	41.7	56.8	58.2	4.9	0.0	7.335886	120692	120771	217-217	1.000000	40.4
trnL_uag_p4	trnL_uag	4	TGAATTGACTTCAATCTCGACGA	GGGTTTCTCGATTTTATGGAATGG	23	24	39.1	41.7	58.2	58.2	4.9	0.0	7.362785	120692	120771	217-217	1.000000	40.4
trnL_uag_p5	trnL_uag	5	GAATTGACTTCAATCTCGACGA	GAGGGTTTCTCGATTTTATGGA	22	22	40.9	40.9	56.6	55.9	4.9	0.0	7.542536	120692	120771	218-218	1.000000	40.4
trnN_guu-trnR_acg_p1	trnN_guu-trnR_acg	1	CCGTTGTTCCTCAGTAGCTC	AACAGATCCAGTGGAGACGA	20	20	55.0	50.0	58.0	58.1	0.0	18.7	0.077600	136608	137261	831-831	1.000000	79.5
trnN_guu-trnR_acg_p2	trnN_guu-trnR_acg	2	TGGGTTGAGAACGGGAATTG	AACAGATCCAGTGGAGACGA	20	20	50.0	50.0	58.1	58.1	0.0	18.7	0.170982	136608	137261	872-872	1.000000	78.7
trnN_guu-trnR_acg_p3	trnN_guu-trnR_acg	3	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	136608	137261	795-795	1.000000	78.4
trnN_guu-trnR_acg_p4	trnN_guu-trnR_acg	4	GCTGTTAACTGACTGGTCGT	AACAGATCCAGTGGAGACGA	20	20	50.0	50.0	57.8	58.1	15.9	18.7	0.225666	136608	137261	795-795	1.000000	78.3
trnN_guu-trnR_acg_p5	trnN_guu-trnR_acg	5	TGGTCGTAGGTTCGAATCCT	AACAGATCCAGTGGAGACGA	20	20	50.0	50.0	58.2	58.1	18.9	18.7	0.231901	136608	137261	782-782	1.000000	78.2